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Development and validation of a rapid and accurate ultra performance liquid chromatography-photodiode array method for concurrent quantification of thirty-two polyphenols in edible fruit of Cordia myxa Linn.
J Chromatogr A
December 2024
Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur (Himachal Pradesh), 176061, India; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India. Electronic address:
The aim of current work was to develop a novel, simple, sensitive, and reliable method for screening and quantification of thirty-two polyphenol compounds from Cordia myxa (C. myxa) using Ultra Performance Liquid Chromatography Photodiode Array detector (UPLC-PDA). With the help of the quaternary solvent manager and a comparison study of seven different columns packed with silica particles that are less than two micron thick (1.
View Article and Find Full Text PDFPhytochemical analysis and biological activities of solvent extracts and silver nanoparticles obtained from Woodwardia unigemmata (Makino) Nakai.
PLoS One
January 2025
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
Multidrug resistant bacteria are causing health problems and economic burden worldwide; alternative treatment options such as natural products and nanoparticles have attained great attention recently. Therefore, we aimed to determine the phytochemicals, antibacterial potential, and anticancer activity of W. unigemmata.
View Article and Find Full Text PDFA Protocol for GC-MS Profiling of Chiral Secondary Amino Acids.
Methods Mol Biol
January 2025
Laboratory of Analytical Biochemistry & Metabolomics, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
A simple analytical workflow is described for gas chromatographic-mass spectrometric (GC-MS)-based chiral profiling of secondary amino acids (AAs) in biological matrices. The sample preparation is carried out directly in aqueous biological sample extracts and involves in situ heptafluorobutyl chloroformate (HFBCF) derivatization-liquid-liquid microextraction of nonpolar products into hexane phase followed by subsequent formation of the corresponding methylamides from the HFB esters by direct treatment with methylamine reagent solution. The (O, N) HFB-butoxycarbonyl-methylamide AA products (HFBOC-MA) are separated on a Chirasil-L-Val capillary column and quantitatively measured by GC-MS operated in selected ion monitoring (SIM) mode.
View Article and Find Full Text PDFRedox and Photochemical Reactivity of Cerium(IV) Carbonate and Carboxylate Complexes Supported by a Tripodal Oxygen Ligand.
Inorg Chem
January 2025
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China.
The protonolysis and redox reactivity of a Ce(IV) carbonate complex supported by the Kläui tripodal ligand [(η-CH)Co{P(O)(OEt)}] (L) have been studied. Whereas treatment of [Ce(L)(CO)] () with RCOH afforded [Ce(L)(RCO)] ( = Me (), Ph (), 2-NOCH ()), the reaction of with PhCHCOH resulted in formation of a mixture of Ce(IV) () and Ce(III) () carboxylate species. In benzene in the dark, was slowly converted into via Ce(IV)-O(carboxylate) homolysis.
View Article and Find Full Text PDFPotential Antibacterial of Leaf Sirih Merah Against Enterococcus Faecalis ATCC 29212 Bacteria.
Comb Chem High Throughput Screen
January 2025
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia.
Background: Dental root canal failure is a disease caused by gram-positive bacteria, Enterococcus faecalis. The disease is caused by the bacterial cell wall consisting of a peptidoglycan layer that protects the bacteria from internal osmotic pressure. Peptidoglycan biosynthesis includes many enzymes, such as MurA, Penicillin-binding protein (PBP), and SrtA.
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