AI Article Synopsis
- Researchers created a genetically modified luciferase from Photinus pyralis that produces over 10 times more luminescence than the original enzyme.
- This enhanced luciferase allows detection of ATP at extremely low levels (10^-18 mol), similar to the amount in a single bacterial cell.
- As a result, the team can identify bacterial contamination in samples with as few as one colony-forming unit (c.f.u.) for bacteria like Escherichia coli and Bacillus subtilis.
Article Abstract
Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained in a single bacterial cell. Consequently,we have been able to detect bacterial contamination in samples as low as one colony-forming unit (c.f.u.) for both Escherichia coli and Bacillus subtilis.
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| http://dx.doi.org/10.1007/s10529-008-9643-3 | DOI Listing |
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