Influence of proteins from the Agkistrodon blomhoffii ussuriensis snake venom on platelet activation and aggregation was developed on different model systems in vitro. It was shown that novel disintegrin (Blomus-B) and phospholipase A2 (Blopholipase) from the venom, activated platelets and inhibited their aggregation. Fibrino(geno)lityc enzyme (Blomulyse) does not activate platelets and has no effect on their aggregation stimulated by collagen, but inhibit ADP and adrenalin-stimulated platelet aggregation. Thrombin-like enzyme (Ancistron-Bu) activates platelets but has no effect on their aggregation. Obtained proteins can be used under development of new antiplatelet agents and as instruments for detailed elaboration and deep investigation of processes which proceed with participation of platelets.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effect of venom (AAVC-I) on apoptosis through modulation of the Keap1/Nrf2 pathway in HSC-3 oral squamous cell carcinoma cells.
Transl Cancer Res
August 2024
School of Stomatology, Wannan Medical College, Wuhu, China.
Background: Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the oral and maxillofacial regions. Patients with OSCC exhibit a poor response to conventional chemoradiotherapies, which are associated with severe side effects. Therefore, it is essential to identify an effective therapeutic method to treat patients with OSCC.
View Article and Find Full Text PDFBlood Lines: Intraspecific and Interspecific Variations in Anticoagulant Actions of Viperid Venoms.
Toxins (Basel)
June 2024
Adaptive Biotoxicology Lab, School of the Environment, University of Queensland, St Lucia, QLD 4072, Australia.
This study investigated the intraspecific and interspecific variability in the venom effects of viperid snake species and subspecies (eleven venoms total) on plasma clotting times, fibrinogen levels, and fibrin clot strength. Significant delays in plasma clotting time were observed for , , , , , and . Notably, the phylogenetically disjunct lineages , , and exhibited the most potent anticoagulant effects, indicating the independent amplification of a basal trait.
View Article and Find Full Text PDFDisintegrin Accutin inhibits A549 cell migration though suppression of EMT and FAK/AKT signaling pathway.
Int J Biol Macromol
August 2024
The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China; Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Changsha, Hunan 410081, China; Institute of Interdisciplinary Studies, Hunan Normal University, Changsha 410081, China. Electronic address:
Integrins are heterodimers composed of two subunits, α(120-185kD) and β (90-110kD), which mediate the connection between cells and their external environment, such as extracellular matrix (ECM), and play an important role in the regulation of cell shape, proliferation and migration. Herein, we identified a potent anti-tumor migration peptide Accutin from crude venom of Agkistrodon acutus using an A549 3D tumor sphere model, and simulation tools and RNA sequencing were performed to reveal the mechanism of Accutin. Accutin is a disintegrin and docking, molecular dynamics simulations and ITC assay indicate that the RGD motif in the Accutin sequence can stably bind to integrins α5β1.
View Article and Find Full Text PDFIncidence, clinical features, and risk factors of hemocoagulase-induced hypofibrinogenemia: A retrospective real-world study.
Medicine (Baltimore)
April 2024
Department of Clinical Pharmacy, Weifang People's Hospital, Kuiwen District, Weifang, Shandong Province, P. R. China.
The objective of this study was to explore the real-world incidence, severity, clinical features, and potential risk factors associated with hypofibrinogenemia induced by hemocoagulase. Based on Chinese Hospital Pharmacovigilance System, a retrospective case-control study was conducted, enrolling hospitalized patients who received hemocoagulase for the treatment or prevention of hemorrhage in Weifang People's Hospital in China from January 2021 to May 2022. Univariate and multivariate logistic regression was performed to analyze the potential risk factors.
View Article and Find Full Text PDFComments on "Comparing cost of F(ab')AV vs FabAV in the treatment of copperhead envenomation".
Am J Emerg Med
February 2024
Division of Medical Toxicology, Department of Emergency Medicine, Washington University School of Medicine, Saint Louis, MO 63110, United States of America.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!