AI Article Synopsis
- Developed a bioassay using bioconjugated dye-doped silica nanoparticles (NPs) to accurately detect a single bacterial cell in 20 minutes without amplification.
- The antibody-conjugated NPs specifically detect bacteria like Escherichia coli O157:H7 in beef by leveraging antibody-antigen interactions.
- This technology shows promise for broad applications in biotechnology and medicine, aiming to enhance detection of disease markers and infectious agents.
Article Abstract
Using bioconjugated dye-doped silica nanoparticles (NPs), we have developed a bioassay for the accurate determination of a single bacterial cell within 20 minutes without any signal amplification or sample enrichment. The antibody-conjugated NPs can specifically and quantitatively detect bacteria, such as Escherichia coli O157:H7 from beef through antibody-antigen recognition. Dye-doped silica NPs have also been successfully used for DNA detection at sub-fentomolar concentrations. Our results demonstrate the potential of dye-doped silica NPs for broad applications in practical biotechnological and medical applications in various biodetection systems. The ultimate goal of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents.
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| http://dx.doi.org/10.1007/978-0-387-76713-0_10 | DOI Listing |
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