We discuss how actively-driven optical tweezers may be used to characterize Brownian microparticles. Two experiments are described in detail. We follow the thermal fluctuations of a charged particle in an oscillatory electric field and demonstrate that charges as low as a few elementary charges can be measured accurately and reproducibly. Secondly, we measure the orientational dynamics of a trapped rotating droplet and use circular polarimetry within optical tweezers to determine in situ birefringence.

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http://dx.doi.org/10.1039/b703994hDOI Listing

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