AI Article Synopsis
- A yeast two-hybrid screening was conducted to find proteins that interact with cardiac troponin I-interacting kinase (TNNI3K), aiming to understand its regulatory roles.
- The study identified antioxidant protein 1 (AOP-1) as an interaction partner of TNNI3K, which was confirmed through binding assays and coexpression experiments.
- Confocal immunofluorescence showed that TNNI3K and AOP-1 colocalize, and AOP-1 was found to inhibit the kinase activity of TNNI3K in vitro.
Article Abstract
In the present study, a yeast two-hybrid screening system was used to identify the interaction partners of cardiac troponin I-interacting kinase (TNNI3K) that might serve as regulators or targets, and thus in turn to gain some insights on the roles of TNNI3K. After screening the adult heart cDNA library with a bait construct encoding the ANK motif of TNNI3K, antioxidant protein 1 (AOP-1) was isolated. The interaction between TNNI3K and AOP-1 was confirmed by the in vitro binding assay and coexpression experiments in vivo. The colocalization of TNNI3K and AOP-1 was clarified by confocal immunofluorescence. Moreover, coexpression of AOP-1 inhibited TNNI3K kinase activity in the in vitro kinase assay.
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| http://dx.doi.org/10.1134/s0006297907110053 | DOI Listing |
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