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Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification.
Bio Protoc
December 2022
Dept of Musculoskeletal and Ageing Science, University of Liverpool, Liverpool, United Kingdom.
Immunoglobulins are proteins produced by the immune system, which bind specifically to the antigen that induced their formation and target it for destruction. Highly purified human immunoglobulins are commonly used in research laboratories for several applications, such as in vitro to obtain hybridomas and in vivo animal immunisation. Several affinity purification methods are used to purify immunoglobulins from human serum, such as protein A/G Sepharose beads, polyethylene glycol, and caprylic acid ammonium sulphate precipitation.
View Article and Find Full Text PDFProspective randomized controlled blinded clinical trial evaluating biomarkers of acute kidney injury following 6% hydroxyethyl starch 130/0.4 or Hartmann's solution in dogs.
J Vet Emerg Crit Care (San Antonio)
May 2021
College of Science, Health, Engineering and Education, Murdoch University, Perth, Western Australia, Australia.
Objective: To evaluate the effect of 6% hydroxyethyl starch (HES) 130/0.4, compared with a Hartmann's solution control (CRYST), on urine biomarkers of acute kidney injury (AKI) in dogs prescribed a fluid bolus.
Design: Randomized, controlled, blinded clinical trial January 2018 to February 2019.
The effect of simulated inflammatory conditions on the surface properties of titanium and stainless steel and their importance as biomaterials.
Mater Sci Eng C Mater Biol Appl
September 2016
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, México.
This work compares the surface modifications induced by the immersion in solutions that simulate inflammatory conditions of pure titanium (cpTi) and medical grade stainless steel (SS). The inflammatory conditions were simulated using a mixture of Hartman solution and 50mM of hydrogen peroxide (H2O2) at pH=5.2.
View Article and Find Full Text PDFHyperemesis in Pregnancy Study: a pilot randomised controlled trial of midwife-led outpatient care.
Eur J Obstet Gynecol Reprod Biol
May 2016
Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. Electronic address:
Objective: To assess the feasibility of implementing a complex intervention involving rapid intravenous rehydration and ongoing midwifery support as compared to routine in-patient care for women suffering from severe nausea and vomiting in pregnancy, (NVP)/hyperemesis gravidarum (HG).
Study Design: 53 pregnant women attending the Maternity Assessment Unit (MAU), Newcastle upon Tyne NHS Foundation Trust, Newcastle, UK with moderate-severe NVP, (as determined by a Pregnancy Unique Quantification of Emesis and Vomiting [PUQE] score ≥nine), consented to participate in this pilot randomised controlled trial (RCT). Subsequently 27 were randomised to the intervention group, 26 to the control group.
Hyponatraemic encephalopathy in azotaemic neonatal foals: four cases.
Aust Vet J
December 2014
Goulburn Valley Equine Hospital, Congupna, Victoria, Australia.
Case Series: Four neonatal foals were presented, over a 2-year period, (2011-2012) with aimlessly walking, head pressing, 'chewing gum' seizures and ataxia. The neurological lesion was consistent with increased intracranial pressure in all cases. All foals had severe hyponatraemia and azotaemia identified on biochemistry.
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