AI Article Synopsis
- The study investigates the expression and distribution of aquaporin 3 (AQP3) and aquaporin 9 (AQP9) in the colonic mucosa of patients with functional constipation, comparing it to a control group.
- Immunohistochemistry and Western blotting techniques revealed that AQP3 was more prevalent in the ascending colon of constipated patients, while AQP9 showed reduced expression in the descending colon.
- The findings suggest that AQP3 and AQP9 potentially contribute to the mechanisms underlying functional constipation.
Article Abstract
Objective: To study the expression and distribution of aquaporin 3 (AQP3) and aquaporin 9 (AQP9) in colonic mucosa of patients with functional constipation, and to examine the relationship of constipation with AQP3 and AQP9.
Methods: Immunohistochemistry and semi-quantitative Western blotting were used to detect the expression and distribution of AQP3 and AQP9 in colonic mucosa of 45 patients with functional constipation (trial group) and 21 cases without constipation (control group). Gray scale ratios of AQP3 and AQP9 to beta-actin protein as interior reference were relative amounts of AQP3 and AQP9.
Results: Immunohistochemistry showed that AQP3 was distributed mainly in basement and cavosurface membrane of epithelial cell of colonic mucosa and AQP9 mainly in basement membrane of goblet cell in cavosurface colonic mucosa. Western blotting revealed that the average values of gray scale ratios of AQP3 in ascending colon of trial group and control group were 0.905 and 0.798 (P<0.05),while those of AQP9 were 0.544 and 0.543 (P>0.05), respectively. The average values of gray scale ratios of AQP3 in descending colon of trial group and control group were 0.697 and 0.701 (P>0.05), while those of AQP9 were 0.575 and 0.732 (P<0.05), respectively.
Conclusions: Up-regulated expression of AQP3 in ascending colon and down-regulated expression of AQP9 in descending colon are presented in patients with functional constipation as compared to patients without functional constipation. AQP3 and AQP9 may play a significant role in the onset and development of constipation.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Introduction: Gastroenterocolitis is one of the adverse events related to immune checkpoint inhibitors. However, inflammation of the intestinal lesion used for urinary diversion is not well known as an adverse event related to their use.
Case Presentation: A patient with metastatic bladder cancer was administered pembrolizumab as second-line treatment.
Foreign body ingestion in children: Clinical features and complications.
Tunis Med
December 2024
Department of Pediatric Medicine B, Béchir Hamza Children's Hospital, Faculty of Medicine of Tunis, Tunis el Manar University, Tunis, Tunisia.
Introduction: The ingestion of foreign body (FB) is a common problem in paediatrics. Children are curious by nature and tend to explore environment by inserting objects into their mouths.
Aim: To update our epidemiological and clinical data and adapt clinical management in order to limit morbidity associated with this fairly frequent accidental pathology.
Successful Re-ileostomy Using Skin Flap Formation Techniques.
Cureus
December 2024
Department of Surgery, Kishiwada Tokushukai Hospital, Kishiwada, JPN.
A 61-year-old woman underwent an emergent operation with sigmoid colon cancer resection, colostomy, and ileostomy on colon perforation. The low ileostoma, caused by intra-abdominal bad conditions, had irritated the surrounding skin after surgery, intermittently forcing the patient to fast for a certain period. Six months after the operation, under the judgment that re-ileostomy, essential for hospital discharge, seemed very difficult through another laparotomy, we attempted to make the ileostoma higher not with pulling the ileum from the abdomen but with lowering the surrounding skin using skin flap formation techniques.
View Article and Find Full Text PDFIloprost-Loaded Electrospun Polycaprolactone/Gelatin Membranes for Enhanced Healing of Colon Anastomosis.
J Biomed Mater Res B Appl Biomater
January 2025
Department of Biomedical Engineering, TOBB Economy and Technology University, Ankara, Türkiye.
Despite the variety of proposed solutions, anastomotic leakage is still a critical complication after colorectal surgery, which causes increased clinical mortality and morbidity. By enhancing microcirculation in the colonic mucosa, the use of Iloprost (Ilo) has shown promising results for the healing of anastomosis. The purpose of this study is to examine the performance of Ilo-impregnated Polycaprolactone:Gelatin electrospun membranes (PCL/Gel/Ilo) on anastomosis repair and intra-abdominal adhesion behavior in the Rat colon.
View Article and Find Full Text PDFSLC26A3 (DRA, the Congenital Chloride Diarrhea Gene): A Novel Therapeutic Target for Diarrheal Diseases.
Cell Mol Gastroenterol Hepatol
December 2024
- Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois, Chicago, IL, USA; - Jesse Brown VA Medical Center, Chicago, IL, USA. Electronic address:
Diarrhea associated with enteric infections, gut inflammation, and genetic defects poses a major health burden and results in significant morbidity and mortality. Impaired fluid and electrolyte absorption and/or secretion in the intestine are the hallmark of diarrhea. Electroneutral NaCl absorption in the mammalian GI tract involves the coupling of Na/H and Cl/HCO exchangers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!