A method based on ultrasonic extraction (USE) followed by LC-MS is presented for the determination of vitexin and isovitexin in pigeonpea extracts in this study. The influential parameters of the USE procedure were optimized, and the optimal conditions were as follows: extraction solvent, 60% ethanol solution; liquid/solid ratio 10:1 (mL/g), extraction power, 250 W; temperature, 40-50 degrees C; and three extraction cycles, each cycle 15 min. Validation of the USE method was performed in terms of repeatability and reproducibility. RSDs for extraction yields were lower than 5.85 and 8.09%, respectively. The LOD and LOQ of chromatographic determination were 0.96 and 3.2 ng/mL for vitexin and 0.84 and 2.8 ng/mL for isovitexin. The method was also successfully applied for the determination of vitexin and isovitexin in stems, leaves, and root extracts of pigeonpea. From all these results, we may conclude that the developed method is appropriate for the quality control of pigeonpea and other plant extract products developed from pigeonpea.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jssc.200700312 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Polyphenol metabolomics reveals the applications and prospects of polyphenol-rich plants in natural dyes.
For Res (Fayettev)
December 2024
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Polyphenols, as one of the primary compounds produced by plant secondary metabolism, have garnered considerable attention because of their non-toxic, environmentally friendly, and biodegradable properties, as well as their notable medicinal value. This study presents a metabolomic analysis of polyphenols from 11 woody plants, including , , and , investigating a total of 40 polyphenolic metabolites. A differential metabolite dynamics map highlighted the five most differentiated substances among the 11 plants, including vitexin, dihydromyricetin, genistin, resveratrol, and isorhamnetin.
View Article and Find Full Text PDFThe effect of supplemental LED lighting in the range of UV, blue, and red wavelengths at different ratios on the accumulation of phenolic compounds in pak choi and swiss chard.
Food Res Int
January 2025
Division Quality and Sensory of Plant Products, Georg-August-Universität Göttingen, Carl-Sprengel-Weg 1, 37075 Göttingen, Germany. Electronic address:
Phenolic compounds are known for their health-promoting effects on humans. Pak choi (Brassica rapa ssp. chinensis) and Swiss chard (Beta vulgaris subsp.
View Article and Find Full Text PDFVitexin alleviates MNNG-induced chronic atrophic gastritis via inhibiting NLRP3 inflammasome.
J Ethnopharmacol
January 2025
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR China. Electronic address:
Ethnopharmacological Relevance: The dried fruit of Crataegus pinnatifida Bunge (Hawthorn in Chinese) is a traditional medicine used in China, Japan and Korea for thousands of years. Hawthorn is documented in the Chinese Pharmacopoeia, as a folk medicine that is used to eliminate food, strengthen the stomach, move qi and dissipate blood stasis, treat stagnation of meat and food, gastric distention and fullness, and has anti-inflammatory effects. Vitexin, a flavonoid glycoside, is an important biologically active compound derived from Hawthorn, with significant antioxidant and anti-inflammatory properties.
View Article and Find Full Text PDFDeciphering Structural Dynamics of Atherosclerosis Proteins: Insights from Phytochemicals that Interceded Functional and Structural Changes in Targeted Atherosclerotic Proteins.
ACS Omega
December 2024
Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, Tamilnadu 620024, India.
Atherosclerosis (ASC) is characterized by foam cell-mediated plaque formation, vascular endothelial inflammation, and lipidosis and is the rudimentary cause of cardiovascular diseases. This is the pre-eminent global factor of mortality. This etiological paradigm is significantly influenced by several proteins, where 23 pivotal proteins involved in ASC were meticulously gleaned on the basis of literature studies.
View Article and Find Full Text PDFUnraveling the potential of Epicatechin gallate from crataegus oxyacantha in targeting aberrant cardiac Ca2+ signalling proteins: an in-depth investigation for heart failure therapy.
J Biomol Struct Dyn
December 2024
Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, Tamilnadu, India.
The cardiovascular sarcoplasmic reticulum (SR) calcium (Ca2+) ATPase is an imperative determinant of cardiac functionality. In addition, anomalies in Ca2+ handling protein and atypical energy metabolism are inherent in heart failure (HF). Moreover, Ca2+ overload in SR leads to mitochondrial matrix Ca2+ overload, which can trigger the generation of Reactive Oxygen Species (ROS), culminating in the triggering of the Permeability Transition Pore (PTP) and Cytochrome C release, resulting in apoptosis that leads to arrhythmias and numerous disorders.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!