AI Article Synopsis
- Simultaneous identification and analysis of perfect and imperfect microsatellites helps clarify definitions of simple sequence repeats (SSRs) and their evolutionary history.
- A study on four E. coli and seven Chlamydial strains shows that Chlamydial strains have a higher abundance and greater variation in SSR distribution compared to E. coli.
- Interestingly, while most genomes exhibit similar patterns in trinucleotide repeat lengths, the unique distribution in C. muridarum indicates a faster evolution of SSRs in that strain, highlighting the significance of microsatellites in prokaryotic genome evolution.
Article Abstract
Simultaneous identification and comparison of perfect and imperfect microsatellites within a genome is a valuable tool both to overcome the lack of a consensus definition of SSRs and to assess repeat history. Detailed analysis of the overall distribution of perfect and imperfect microsatellites in closely related bacterial taxa is expected to give new insight into the evolution of prokaryotic genomes. We have performed a genome-wide analysis of microsatellite distribution in four Escherichia coli and seven Chlamydial strains. Chlamydial strains generally have a higher density of SSRs and show greater intra-group differences of SSR distribution patterns than E. coli genomes. In most investigated genomes the distribution of the total lengths of matching perfect and imperfect trinucleotide repeats are highly similar, with the notable exception of C. muridarum. Closely related strains show more similar repeat distribution patterns than strains separated by a longer divergence time. The discrepancy between the preferred classes of perfect and imperfect repeats in C. muridarum implies accelerated evolution of SSRs in this particular strain. Our results suggest that microsatellites, although considerably less abundant than in eukaryotic genomes, may nevertheless play an important role in the evolution of prokaryotic genomes and several gene families.
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| http://dx.doi.org/10.1016/j.gene.2007.11.006 | DOI Listing |
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