AI Article Synopsis

  • The Bacillus cereus group includes species like Bacillus anthracis and Bacillus thuringiensis, recognized for their large plasmids that contribute to virulence.
  • A new protocol is introduced for isolating and detecting large plasmids, capable of identifying those up to 350kb in size.
  • The study also reveals that Bacillus mycoides possesses large plasmids, expanding knowledge about plasmid distribution in this bacterial group.

Article Abstract

The members of the Bacillus cereus group, Bacillus anthracis, Bacillus thuringiensis, and B. cereus senso stricto, are largely defined by their content of large plasmids, which encode major virulence factors. Here we offer an easy, fast, and reliable protocol for the isolation and detection of large plasmids up to the size of at least 350kb. Furthermore, using this method, we report that Bacillus mycoides contain large plasmids.

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http://dx.doi.org/10.1016/j.plasmid.2007.11.005DOI Listing

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