AI Article Synopsis
- There is growing interest in developing new methods to effectively measure the redox state and image living cells at a high resolution.
- Researchers used tiny amperometric probes with a scanning electrochemical microscope (SECM) to conduct experiments on cultured human breast cells, achieving minimal damage to the cell membrane.
- The technique allowed for detailed measurements of charge transport, membrane potential, and redox properties at a subcellular level, as well as imaging the surface of the cells.
Article Abstract
There is a significant current interest in development of new techniques for direct characterization of the intracellular redox state and high-resolution imaging of living cells. We used nanometer-sized amperometric probes in combination with the scanning electrochemical microscope (SECM) to carry out spatially resolved electrochemical experiments in cultured human breast cells. With the tip radius approximately 1,000 times smaller than that of a cell, an electrochemical probe can penetrate a cell and travel inside it without apparent damage to the membrane. The data demonstrate the possibility of measuring the rate of transmembrane charge transport and membrane potential and probing redox properties at the subcellular level. The same experimental setup was used for nanoscale electrochemical imaging of the cell surface.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206555 | PMC |
| http://dx.doi.org/10.1073/pnas.0711075105 | DOI Listing |
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