Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Aim: To establish a novel method for antibody analysis based on visual protein microarray.
Methods: The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.
Results: The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.
Conclusion: This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.
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