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[Method of antibody analysis based on visual protein array]. | LitMetric

[Method of antibody analysis based on visual protein array].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi

College of Life Science, Capital Normal University, Beijing 100037, China.

Published: January 2008

Aim: To establish a novel method for antibody analysis based on visual protein microarray.

Methods: The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.

Results: The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.

Conclusion: This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.

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