AI Article Synopsis
- A new cross-linking reagent, octadiazopyruvoyl PAMAM (8G.1 DAP), was created by modifying first-generation polyamidoamine dendrimer with p-nitrophenyl diazopyruvate.
- Upon exposure to light above 300 nm in methanol, this compound undergoes Wolff rearrangements, producing methyl esters from ketenes.
- The reagent demonstrated strong bonding capabilities with dehydrated collagen, achieving tensile strengths of up to 36 N cm(-2) for collagen-glass bonds and 92 N cm(-2) for collagen-collagen bonds, though bonding strength diminished significantly with increased tissue hydration.
Article Abstract
A new cross linking reagent based on the first-generation polyamidoamine dendrimer (G.1 PAMAM) has been synthesized by reaction of the PAMAM with eight equivalents of p-nitrophenyl diazopyruvate. The resulting water-soluble octadiazopyruvoyl PAMAM (8G.1 DAP, 1.3) was shown to undergo Wolff rearrangements upon photolysis in methanol at lambda > 300 nm to yield the methyl esters of the ketenes formed from the loss of nitrogen. 8G.1 DAP also forms strong bonds with dehydrated collagen with glass as high as 36 N cm(-2). Collagen to collagen bonds with tensile strengths as high as 92 N cm(-2) were observed with fully dehydrated tissues. The bonding decreased rapidly with increasingly hydrated tissue possibly due to the increased distance between the collagen fibrils and the competition of H2O for the free ketene functions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739328 | PMC |
| http://dx.doi.org/10.1111/j.1751-1097.2007.00218.x | DOI Listing |
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