[Extraction of sex hormone from antler velvet with supercritical CO2].

Objective: To study the method of extraction of sex hormone from antler velvet with supercritical CO2.

Method: Supercritical fluid extraction (SFE) was used to extract sex hormone from antler velvet and radioimmunoassay (RIA) was used to analyze the extracts. The chemical compositions in extracts were identified by GC-MS, TLC and HPLC, respectively.

Result: The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 degrees C and extraction pressure of 30 MPa. Estradiol and progesterone in the extracts were 3.07, 776.18 ng x g(-1) respectively.

Conclusion: It is feasible to extract hormone from antler velvet with supercritical CO2.