We investigated the mechanism of H(2)O(2) activation of the Ca(2+)-regulated NADPH oxidase NOX5. H(2)O(2) induced a transient, dose-dependent increase in superoxide production in K562 cells expressing NOX5. Confocal studies demonstrated that the initial calcium influx generated by H(2)O(2) is amplified by a feedback mechanism involving NOX5-dependent superoxide production and H(2)O(2). H(2)O(2) NOX5 activation was inhibited by extracellular Ca(2+) chelators, a pharmacological inhibitor of c-Abl, and overexpression of kinase-dead c-Abl. Transfected kinase-active GFP-c-Abl colocalized with vesicular sites of superoxide production in a Ca(2+)-dependent manner. In contrast to H(2)O(2), the Ca(2+) ionophore ionomycin induced NOX5 activity independent of c-Abl. Immunoprecipitation of cell lysates revealed that active GFP-c-Abl formed oligomers with endogenous c-Abl and that phosphorylation of both proteins was increased by H(2)O(2) treatment. Furthermore, H(2)O(2)-induced NOX5 activity correlated with increased localization of c-Abl to the membrane fraction, and NOX5 proteins could be coimmunoprecipitated with GFP-Abl proteins. Our data demonstrate for the first time that NOX5 is activated by c-Abl through a Ca(2+)-mediated, redox-dependent signaling pathway and suggest a functional association between NOX5 NADPH oxidase and c-Abl.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2278123 | PMC |
| http://dx.doi.org/10.1016/j.freeradbiomed.2007.11.020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis.
BMC Plant Biol
January 2025
Institute of Food Crops, Hubei Academy of Agricultural Sciences/Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, 430064, China.
Background: Sucrose non-fermenting-1-related protein kinases (SnRKs) have been implicated in plant growth and stress responses. Although SnRK3.23 is known to be involved in drought stress, the underlying mechanism of resistance differs between Arabidopsis and rice, and little is known about its function in wheat.
View Article and Find Full Text PDFReplacement of fishmeal with Quinoa Husk (Chenopodium quinoa) for mitigating multiple stresses in Pangasianodon Hypophthalmus.
Sci Rep
January 2025
ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, 413115, India.
The fishmeal is boon for aquaculture production in this recent pollution and climate change era. However, the demand of fishmeal is enhancing in many folds which needs to find alternative to fishmeal in cheap price. The present investigation addresses these issues with quinoa husk (QH).
View Article and Find Full Text PDF(-)-Epigallocatechin-3-gallate promotes the dermal papilla cell proliferation and migration through the induction of VEGFA.
Biochim Biophys Acta Mol Cell Res
January 2025
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China. Electronic address:
Dermal papilla cells (DPCs) are crucial for the growth and development of hair follicles (HF). (-)-Epigallocatechin-3-gallate (EGCG) is the primary catechin identified in green tea, which has antioxidant effects and regulates cell activity. This study demonstrates that EGCG could promote the proliferation of DPCs.
View Article and Find Full Text PDFEffects of drinking water supplemented with apple vinegar, essential oils, or colistin sulfate on growth performance, blood lipids, antioxidant status, intestinal morphology, and gut microflora of broiler chickens.
Poult Sci
January 2025
School of Agriculture and Food Sustainability, The University of Queensland, Gatton Campus, Gatton, QLD 4343, Australia; Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 49189-4364, Iran.
With rising concerns about antibiotic resistance and its consequences on public health, the identification of safe and effective alternatives to antibiotics in the poultry industry has become increasingly critical. This study aimed to evaluate the effects of supplementing drinking water with apple vinegar and essential oils, compared to an antibiotic growth promoter, on the growth performance, serum lipid profile, antioxidant status, intestinal morphology, and gastrointestinal microflora population of broiler chickens. A total of 240 one-day-old male broiler chickens were randomly assigned to four treatments, each consisting of six replicate pens with ten birds per pen.
View Article and Find Full Text PDFHigh performance ozone nanobubbles based advanced oxidation processes (AOPs) for degradation of organic pollutants under high pollutant loading.
J Environ Manage
January 2025
Department of Sanitary Engineering, Faculty of Civil and Environmental Engineering, Gdansk University of Technology, Narutowicza 11/12 Str., 80-233, Gdansk, Poland; School of Civil, Environmental, and Architectural Engineering, College of Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea. Electronic address:
Advanced Oxidation Processes (AOPs) have proven to be an effective solution for chemical wastewater treatment, particularly for degradation of organic pollutants, especially dyes. Ozonation is recognized as one of the most prevalent AOPs. Nevertheless, some cases show a lowered efficiency of O utilization which is attributed to its inadequate distribution in the treated water causing low residence time, low mass transfer coefficient as well as shorter half-life.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!