AI Article Synopsis
- Researchers developed a rapid immunochromatography (ICG) strip test to detect aflatoxin B1 (AFB1) using a nanocolloidal gold-antibody probe, achieving a visual detection limit of 0.5 ng/ml.
- The test was designed with a specific monoclonal antibody that reacts not only to AFB1 but also to related aflatoxins B2, G1, and G2.
- Results from the ICG test were consistent with traditional HPLC analysis across 172 grain and feed samples, showing its potential as a fast, cost-effective screening method for mycotoxins in food and agricultural products, with results deliverable in just 15 minutes.
Article Abstract
An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.
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