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Biosynthesis of C14-labeled amino acids by Chlorella ellipsoidae.
J Gen Appl Microbiol
December 2004
Division of Enzymology, The Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan.
Domain formation in sphingomyelin/cholesterol mixed membranes studied by spin-label electron spin resonance spectroscopy.
Biochemistry
March 2005
Departamento de Bioquímica, Universidad del País Vasco, Apartado 644, E-48080 Bilbao, Spain.
Interactions of palmitoylsphingomyelin with cholesterol in multilamellar vesicles have been studied over a wide range of compositions and temperatures in excess water by using electron spin resonance (ESR) spectroscopy. Spin labels bearing the nitroxide free radical group on the 5 or 14 C-atom in either the sn-2 stearoyl chain of phosphatidylcholine (predominantly 1-palmitoyl) or the N-stearoyl chain of sphingomyelin were used to determine the mobility and ordering of the lipids in the different phases. Two-component ESR spectra of the 14-position spin labels demonstrate the coexistence first of gel (L(beta)) and liquid-ordered (L(o)) phases and then of liquid-ordered and liquid-disordered (L(alpha)) phases, with progressively increasing temperature.
View Article and Find Full Text PDFIntestinal permeability to small- and large-molecular-weight substances in the newborn rabbit.
J Pediatr Surg
October 1997
Section of Pediatric Surgery, C.S. Mott Children's Hospital and the University of Michigan Medical School, Ann Arbor 48109, USA.
Background/purpose: The authors have previously reported the occurrence of spontaneous bacterial translocation (BT) and its resolution with age in the newborn rabbit. They have also reported a close correlation between small bowel bacterial colonization (BC-SB) and BT at 1 week of age, suggesting that the presence of luminal bacteria and their production of endotoxins may increase the intestinal permeability. The aim of this study was to evaluate intestinal permeability to small and large molecules in the newborn rabbit and to correlate it with BT.
View Article and Find Full Text PDFThe transport of vitamin C in the isolated human near-term placenta.
Eur J Obstet Gynecol Reprod Biol
September 1995
Abteilung für experimentelle Medizin, Universitäts-Frauenklinik Eppendorf, Hamburg, Germany.
We investigated the specific uptake (reference: [H3]-L-glucose) as a measure of membrane transfer of [C14]-labeled L-ascorbic acid (AA), L-dehydroascorbic acid (DHA) and diketogulonic acid using the single injection, double tracer dilution method in the artificially perfused lobe of the near-term human placenta. The uptake of DHA (40-60%) on both the fetal and the maternal side was 3-6 times higher than the uptake of AA, whereas an uptake of diketogulonic acid was not detected. AA transport was slightly higher on the maternal side.
View Article and Find Full Text PDFRegional chemotherapy in the canine liver.
J Surg Oncol
June 1994
Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, New York.
Using a fluorometric assay, mean drug levels of Adriamycin were significantly higher in the normal canine liver following bolus injection of this drug via the hepatic artery or portal vein as compared to hepatic tissue levels following systemic intravenous administration (P < 0.001). However, the variations in tissue levels observed after hepatic artery (+/- 16.
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