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Chiral N-isobutyryl-cysteine protected gold nanoparticles: preparation, size selection, and optical activity in the UV-vis and infrared.
J Am Chem Soc
August 2006
Université de Neuchâtel, Institut de Microtechnique, Rue Emile-Argand 11, 2009- Neuchâtel, Switzerland.
We have prepared gold nanoparticles covered with N-isobutyryl-l-cysteine and N-isobutyryl-d-cysteine, respectively. These particles with a mean particle size smaller than 2 nm are highly soluble in water and are amenable to chiroptical techniques such as vibrational circular dichroism (VCD) and circular dichroism (CD) spectroscopy. Density functional theory shows that the VCD spectra are sensitive toward the conformation of the adsorbed thiol.
View Article and Find Full Text PDFMilk fat content measurement by a simple UV spectrophotometric method: an alternative screening method.
J Dairy Sci
February 2005
Departamento de Química Biológica-CIQUIBIC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
We developed a simple microtechnique to measure lipids in milk by UV spectrophotometry. This technique is based upon the property of fatty acids to absorb UV light proportional to their concentration. Samples of powdered or fluid milk (30 or 60 muL) were added to 3 mL of analytic grade ethanol and stored at -20 degrees C for at least 1 h.
View Article and Find Full Text PDFFingerprinting of molecular components in individual human cerebrospinal fluid samples with a new micropurification system.
J Chromatogr
July 1991
Department of Pharmacology, University of Uppsala, Sweden.
This paper reports a rapid and sensitive microtechnique for fingerprinting as little as 20 microliters of cerebrospinal fluid (CSF) from individual humans. Different molecular components were also isolated from the fluid. The SMART system, a new system optimized for high-recovery micropurification, was used for this purpose.
View Article and Find Full Text PDFThis paper represents a brief review of traditional procedures for the analysis of confiscated drugs and the application of these procedures to the products formed after reductive fragmentation and halogen-acetylated derivatization of the drugs. Confiscated pills, already tentatively identified by some previous procedure, were analyzed by the use of infrared spectroscopy, ultraviolet spectroscopy, and gas chromatography. The same drugs were confirmed by analyzing the alcohols obtained after reductive fragmentation with LiA1H4 as well as the acetylated derivative fractions.
View Article and Find Full Text PDFA new microtechnique was used to determine the nucleic acid content of Chironomus polytene chromosomes. The method based on UV-microspectrophotometric measurement of alkali digested chromosome samples before and after gel filtration through Sephadex microcolumns permits the simultaneous estimation of DNA as well as RNA amount of single chromosomes and nucleoli. The DNA and RNA content of C.
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