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Subclinical Mastitis in Small-Holder Dairy Herds of Gansu Province, Northwest China: Prevalence, Bacterial Pathogens, Antimicrobial Susceptibility, and Risk Factor Analysis.
Microorganisms
December 2024
Key Laboratory of New Animal Drug Project, Gansu Province, Lanzhou 730050, China.
This cross-sectional study assessed the prevalence, bacterial distribution, antimicrobial susceptibility, and potential risk factors associated with subclinical mastitis (SCM) in small-holder dairy herds in Gansu Province, Northwest China. Forty small-holder cow farms were randomly selected from eight cities/counties in six districts of Gansu Province, and a total of = 530 lactating cows were included in this study. SCM prevalence was noted at 38.
View Article and Find Full Text PDFIvermectin Combined With Recombinant Methioninase (rMETase) Synergistically Eradicates MiaPaCa-2 Pancreatic Cancer Cells.
Anticancer Res
January 2025
AntiCancer Inc., San Diego, CA, U.S.A.;
Background/aim: Ivermectin was initially utilized as a veterinary medication, demonstrating efficacy against various parasites. Pancreatic cancer is currently one of the most recalcitrant diseases. The aim of the present study was to demonstrate the synergy of the combination of recombinant methioninase (rMETase) and ivermectin to eradicate human pancreatic cancer cells in vitro.
View Article and Find Full Text PDFA study for the genotoxicity assessment of substances containing probiotic candidates in the in vitro TK6 cell micronucleus test: Influence of low pH conditions and antibiotic supplementation on the test results.
Genes Environ
December 2024
Yakult Central Institute, Yakult Honsha Co., Ltd., 5-11 Izumi, Kunitachi-Shi, Tokyo, 186-8650, Japan.
Background: When assessing the genotoxicity of substances containing probiotic candidates, such as lactic acid-producing bacteria, using the in vitro micronucleus test (MNT), bacterial growth in the test medium may reduce the pH of the medium. The low medium pH is known to induce cytotoxicity and false-positive results. In the TK6 cell system, it is difficult to completely remove the bacteria from the medium by washing post-treatment, leading to bacterial growth during the recovery period in the short-term treatment.
View Article and Find Full Text PDFMagnetic graphene oxide increases the biocompatibility and nuclear factor erythroid 2-related factor 2 antioxidant of human cumulus cells: A lab-trial study.
Int J Reprod Biomed
September 2024
Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Article Synopsis
- Assisted reproductive technology currently has a low success rate of 30%, and improving the survival of cumulus cells (CCs) could enhance the function of oocytes in infertile women.
- The study investigated the effects of magnetic graphene oxide (MGO) nanocomposite on the biocompatibility and antioxidant properties of human CCs collected from 37 women.
- Results indicated that MGO at a concentration of 50 µg/ml increased the viability of CCs and boosted antioxidant protein Nrf2 expression, suggesting potential benefits for in vitro fertilization success.
An efficient serum-free medium for ex vivo expansion of myoblasts from Larimichthys crocea for cultured meat production.
Food Res Int
November 2024
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; Future Food Laboratory, Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China. Electronic address:
The large-scale production of cultured meat has been restricted by high cost of serum, batch difference and unclear composition, thus it is important to develop serum-free media for cell growth. Here we obtained a serum-free medium (SFM), which was suitable for ex vivo culturing of myoblasts from larimichthys crocea. It offered simple composition and high effectiveness that supporting myoblast attachment, expansion and differentiation.
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