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Enzymatic synthesis of β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside using Escherichia coli glycoside phosphorylase YcjT.
Biosci Biotechnol Biochem
September 2023
Graduate School of Bioresources, Mie University, Tsu, Japan.
YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using β-d-glucose 1-phosphate as a donor. The product was identified as β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside.
View Article and Find Full Text PDFGrowth of Bifidobacterium pseudocatenulatum in medium containing N-acetylsucrosamine: enzyme that induces the growth of this bacterium via degradation of this disaccharide.
Glycobiology
May 2022
Bioresource Utilization Science Course, Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Bifidobacterium pseudocatenulatum grows well in the early stages of cultivation in medium containing sucrose (Suc), whereas its growth in medium containing the analogue disaccharide N-acetylsucrosamine (SucNAc) tends to exhibit a considerable delay. To elucidate the cause of this phenomenon, we investigated the proliferation pattern of B. pseudocatenulatum in medium containing D-glucose (Glc) and SucNAc and identified the enzyme that degrades this disaccharide.
View Article and Find Full Text PDFDiscovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose.
Sci Rep
January 2022
Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
Glycoside phosphorylases (GPs), which catalyze the reversible phosphorolysis of glycosides, are promising enzymes for the efficient production of glycosides. Various GPs with new catalytic activities are discovered from uncharacterized proteins phylogenetically distant from known enzymes in the past decade. In this study, we characterized Paenibacillus borealis PBOR_28850 protein, belonging to glycoside hydrolase family 94.
View Article and Find Full Text PDFEnzymatic control and evaluation of degrees of polymerization of β-(1→2)-glucans.
Anal Biochem
November 2021
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
β-(1 → 2)-Glucans can be synthesized by 1,2-β-oligoglucan phosphorylase using β-(1 → 2)-glucooligosaccharides as acceptors and α-d-glucose 1-phosphate as a donor. Using phosphorolysis of sucrose as a source of α-d-glucose 1-phosphate, we generated β-(1 → 2)-glucans with degrees of polymerization (DPs) up to approximately 280. Average DPs up to approximately 1000 were obtained using β-(1 → 2)-glucan with average DP of 160 as an acceptor and pure α-d-glucose 1-phosphate as a donor.
View Article and Find Full Text PDFHigh-Yield Biosynthesis of Glucosylglycerol through Coupling Phosphorolysis and Transglycosylation Reactions.
J Agric Food Chem
December 2020
University of Chinese Academy of Sciences, Beijing 100049, China.
Glucosylglycerol is a powerful osmolyte that has attracted attention as a useful moisturizing ingredient in the cosmetic industry. This study demonstrates two artificially designed synthetic routes for manufacturing glucosylglycerol by combining phosphorolysis and transglycosylation reactions. The overall Gibbs energy change of the synthetic routes was negative, indicating that they are thermodynamically favorable.
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