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It was found that intracellular glycogen is stabilized against acid treatment when it is stored under dry conditions for three months after methanol fixation. This stabilization allowed quantitative double fluorescence staining for nuclear DNA and intracellular glycogen, in a single cell. A Feulgen nucleal reaction, with acriflavine-Schiff's reagent following 5 N HCl hydrolysis at 25 degrees C for 4 min, was followed by a pararosanilin-Schiff PAS reaction for glycogen.
View Article and Find Full Text PDFA prerequisite for automatic image analysis of histoautoradiographs is the arrangement of the silver grains within the same plane of focus. This can be obtained by semi thin sections of 1 micrometer in thickness. A sufficient contrast for the determination of the nuclear areas and the overlying silver grains at the same time can be reached by staining the preparations with Kernechtrot or Feulgen nucleal reaction.
View Article and Find Full Text PDFWe can divide metachrome mordant staining of nuclei after graded 60 degrees C 1 N nitric acid extraction into three groups. The Feulgen nucleal reaction and dilute cationic dye staining of nuclei are abolished in about 30 minutes. With one group of metachrome dyes nuclear staining is lost with acid exposures of one hour or less.
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