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Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking.
Bio Protoc
January 2025
International Institute of Food Innovation Co., Ltd., Nanchang University, Nanchang, China.
PCR-based genome walking is one of the prevalent techniques implemented to acquire unknown flanking genomic DNAs. The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. Therefore, this technique has advanced molecular biology and related fields.
View Article and Find Full Text PDFA Hybrid Rehabilitation Program for Adults with Peripheral Artery Disease (HY-PAD): A Pre-Post Intervention Study to Assess Its Feasibility.
CJC Open
January 2025
Department of Cardiovascular Medicine, Mayo Clinic, Rochester, Minnesota, USA.
Background: Supervised exercise programs improve walking impairment and quality of life (QoL) in patients with peripheral artery disease (PAD). However, such programs are underutilized, due to their limited accessibility. A feasible and effective exercise program is needed.
View Article and Find Full Text PDFEvidence-based exercise recommendations to improve functional mobility in older adults - A study protocol for living systematic review and meta-analysis.
Open Res Eur
January 2025
Neuromotor Behavior and Exercise, University of Münster, Münster, Germany.
Background And Objectives: This is a protocol for a living systematic review and meta-analysis.This review will assess the effects of state-of-the-art exercise interventions designed to promote functional mobility. Therefore, after identifying all potential interventions, we will use the F.
View Article and Find Full Text PDFEffects of Remote Exercise on Physical Function in Pre-Frail Older Adults: A Randomized Controlled Trial.
Med Sci Monit
January 2025
Department of Physical Therapy, Kyungdong University, Gangwon, South Korea.
BACKGROUND Remote exercise have emerged as a promising solution to overcome barriers to physical activity participation in pre-frail older adults, such as limited mobility and accessibility issues. Pre-frail older adults often face barriers to physical activity due to limited mobility and accessibility, underscoring the need for remote exercise alternatives. This study aimed to evaluate and compare the efficacy of remote versus in-person exercise in improving physical function in pre-frail older adults.
View Article and Find Full Text PDFEffect of breathing exercises to prevent pulmonary complications in patients undergoing coronary artery bypass graft surgery: a prospective randomized controlled trials study protocol.
Front Med (Lausanne)
January 2025
Xuanwu Hospital, Capital Medical University, Beijing, China.
Purpose: To study the effects of breathing exercises on preventing pulmonary complications in patients undergoing coronary artery bypass graft surgery.
Methods: Observing whether preoperative breathing exercises can reduce the incidence of postoperative pulmonary complications in patients undergoing coronary artery bypass graft surgery; observing whether these exercises can improve postoperative arterial oxygen pressure, oxygen saturation, and the distance walked in a six-minute walk test after surgery; as well as reduce hospital stay duration, lower treatment costs, and improve the quality of life as measured by the Short Form-36 Health Survey (SF-36).
Design: The study population includes patients undergoing coronary artery bypass graft surgery under general anesthesia; the research center is Capital Medical University Xuanwu Hospital; the sample size is 120.
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