The peptide fragment of H2O2-treated Cu,Zn-superoxide dismutase (SOD) was found to be reactivated with liposomes prepared by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The fragmentation of SOD was observed by 2 mM H2O2 treatment as well as by SOD inactivation and the loss of an alpha-helix in the neighborhood of its activity center. The H2O2-treated SOD, which lost its activity at different incubation times, was dramatically reactivated only by adding POPC liposomes, resulting in 1.3-2.8 times higher enzymatic activity. The ultrafiltration analysis of H2O2-treated SOD co-incubated with liposomes shows that some specific peptide fragments of the oxidized SOD can interact with POPC liposomes. A comparison of the fractions detected in reverse-phase chromatography shows that specific SOD fragments are able to contribute to the reactivation of oxidized and fragmented SOD in the presence of POPC liposomes. The liposomes can recruit the potentially active fragment of SOD among the lethally damaged SOD fragments to elucidate the antioxidative function.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/la702690a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural Dynamics of the Slide Helix of Inactive/Closed Conformation of KirBac1.1 in Micelles and Membranes: A Fluorescence Approach.
J Membr Biol
January 2025
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India.
Inward rectifying potassium (Kir) channels play a critical role in maintaining the resting membrane potential and cellular homeostasis. The high-resolution crystal structure of homotetrameric KirBac1.1 in detergent micelles provides a snapshot of the closed state.
View Article and Find Full Text PDFExploring the Interaction of 3-Hydroxy-4-pyridinone Chelators with Liposome Membrane Models: Insights from DSC and EPR Analysis.
Molecules
December 2024
REQUIMTE, LAQV, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
In this study, we synthesized a series of 3-hydroxy-4-pyridinone (3,4-HPO) chelators with varying lipophilicity by modifying the length of their alkyl chains. To investigate their interaction with lipid membranes, we employed differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy using dimyristoylphosphatidylcholine (DMPC) and palmitoyloleoylphosphatidylcholine (POPC) liposomes as membrane model systems. DSC experiments on DMPC liposomes revealed that hexyl-substituted chelators significantly altered the thermotropic phase behavior of the lipid bilayer, indicating their potential as membrane property modulators.
View Article and Find Full Text PDFEffect of Cholic Acid Salt and Its Mixed Micelles on the Morphology of Giant Unilamellar Vesicles (GUV).
J Oleo Sci
January 2025
Faculty of Science and Technology, Tokyo University of Science.
Article Synopsis
- Bile salts act as biosurfactants in the gastrointestinal tract, helping to emulsify and absorb fat-soluble nutrients and drugs.
- The study utilized giant unilamellar vesicles (GUVs) to investigate the permeation behavior of bile salts and their mixed micelles, using sodium cholate (NaC) and various lipophilic substances.
- Findings showed that below the critical micelle concentration (CMC), NaC causes endocytic changes in GUVs, while above the CMC, mixed micelles interact with the membrane differently, forming aggregates that migrate into the GUV, with variations observed depending on the type of lipophilic component used.
Liposomal Formulations of Novel BODIPY Dimers as Promising Photosensitizers for Antibacterial and Anticancer Treatment.
Molecules
November 2024
Chair and Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Synthesis, photochemical properties, liposomal encapsulation, and in vitro photodynamic activity studies of novel BODIPY dimer connected at positions and its brominated and iodinated analogs were described. UV-Vis measurements indicated that the dimeric structure of obtained BODIPYs did not significantly influence the positions of the absorption maxima. Emission properties and singlet oxygen generation studies revealed a strong heavy atom effect of brominated and iodinated BODIPY dimers, manifested by fluorescence intensity reduction and increased singlet oxygen generation ability compared to analog without halogen atoms.
View Article and Find Full Text PDFCrosstalk of Nucleic Acid Mimics with Lipid Membranes: A Multifaceted Computational and Experimental Study.
Biochemistry
December 2024
LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
Nucleic acid mimics (NAMs) have demonstrated high potential as antibacterial drugs. However, very few studies have assessed their possible diffusion across the bacterial envelope. In this work, we studied NAMs' diffusion in lipid bilayer systems that mimic the bacterial outer membrane using molecular dynamics (MD) simulations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!