Objective: To develop a rapid approach of refractive index detection with high performance liquid chromatography (HPLC) for the determination of rhamnose, fructose, glucose, surose and maltose in Lycium barbarum L.
Methods: The sample was extracted with water and the analyte was separated by Zorbax Carbohydrate column with acetonitrile-water as mobile phase.
Results: Good linear correlations between the concentrations and the peak areas of the analyte were found, with the correlation coefficients ranging from 0.9976 to 0.9998. The spiked recovery rates ranged from 92.27% to 101.93%, with 1.85%-3.27% of relative standard deviations (n=5). The limits of detection (S/N =3) were 4-6 mg/kg.
Conclusion: The proposed method is suitable for the determination of monosaccharide and oligosaccharide in Lycium barbarum L.
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