Objective: To establish a method for detecting methamphetamine (MA) and amphetamine (AMP) with high performance liquid chromatography (HPLC).
Methods: Both MA and AMP were isolated on a C18 column and methanol-phosphate buffer (0.015 mol/L NaH2PO4) at a flow rate of 1.0 mL/min. The 190-360 nm ultraviolet spectrum was examined, with 215 nm as the detection wavelength.
Results: The MA and AMP were well isolated and determined. The MA determined by the HPLC had good linearity with the real value at the range from 1.4 to 270 microg/mL (R2=1), with an average recovery rate of 102.5%. The detectable Limit was 0.73 microg/mL (S/N > or =3). The AMP determined by the HPLC had a good linearity with the real value at the range from 0.9 to 580 microg/mL (R2 = 0.9999), with an average recovery rate of 101.7%. The detectable limit was 0.52 microg/mL (S/N > or =3). Both intra-day and inter-day precisions expressed by relative standard deviations of the MA and AMP were less than 2.4%.
Conclusion: This is a simple, rapid and accurate method for detecting methamphetamine and amphetamine.
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