Objective: To construct the hepatitis B virus (HBV) DNA vaccine pCI/S2S, and study the expression of specific antigen protein in muscle tissue section and the specific immune responses of humoral and cellular immunity in BALB/c mice being inoculated with pCI/S2S.
Methods: DNA templates were acquired from the mixed serum of patients with chronic hepatitis B, and the gene fragment S2S of HBV was cloned. The pCI vector and DNA S2S fragment were doubly cut by Kpn I and Not I, and then linked. HBV DNA vaccine pCI/S2S were intramuscularly injected into BALB/c mice, and boosted after 4 and 8 week. The expressing protein product in muscle tissue in situ and the specific cellular immune response were detected in 12 week after the first injection, the specific humoral immune responses were detected at 1, 2, 4, 6, 8, 12 week after the first injection.
Results: After pCI/S2S intramuscularly injected into BALB/c mice, HBsAb and preS2Ab were gradually determined with cytotoxic T lymphocyte responses induced, and HBsAg protein was expressed in situ in muscle tissue.
Conclusion: pCI/S2S is effective HBV DNA vaccine that can induce the specific humoral and cellular immune responses.
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