A mathematical model of age distribution in the suspension culture was verified. This model used the principle of reverse problems and allowed determination of age distribution from the cell numbers dynamics in the population. The verification was based on the complex non-destructive measurement of the microorganism parameters, which allowed simultaneous determination of the cell population dynamics and the distribution of cells according to their projection areas. The experiments were carried out on E. coli culture.
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Fluids Barriers CNS
January 2025
Department of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital of Southern Medical University, Guangzhou, 510515, China.
Oxidative stress and neuronal apoptosis could be an important factor leading to post-hemorrhagic consequences after germinal matrix hemorrhage (GMH). Previously study have indicated that relaxin 2 receptor activation initiates anti-oxidative stress and anti-apoptosis in ischemia-reperfusion injury. However, whether relaxin 2 activation can attenuate oxidative stress and neuronal apoptosis after GMH remains unknown.
View Article and Find Full Text PDFJ Cardiothorac Surg
January 2025
The First Hospital of Lanzhou University, Lanzhou, China.
Background: This article aims to use high-throughput sequencing to identify miRNAs associated with ferroptosis in myocardial ischemia-reperfusion injury, select a target miRNA, and investigate its role in H9C2 cells hypoxia-reoxygenation injury.
Methods: SD rats and H9C2 cells were used as subjects. ELISA kits quantified MDA, SOD, GSH, LDH, and ferritin levels.
Nature
January 2025
Department of Chemistry, University of Manchester, Manchester, UK.
Cells display a range of mechanical activities generated by motor proteins powered through catalysis. This raises the fundamental question of how the acceleration of a chemical reaction can enable the energy released from that reaction to be transduced (and, consequently, work to be done) by a molecular catalyst. Here we demonstrate the molecular-level transduction of chemical energy to mechanical force in the form of the powered contraction and powered re-expansion of a cross-linked polymer gel driven by the directional rotation of artificial catalysis-driven molecular motors.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Operations Analytics, School of Business and Economics, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Integrating Supply Chain Optimization (SCO) with Life Cycle Assessment (LCA) is essential for creating supply chains that are both economically efficient and environmentally sustainable. While SCO focuses on optimizing network structures and decisions related to product and service delivery, LCA systematically assesses the environmental impacts across the entire supply chain. The existing literature treats SCO and LCA as separate, sequential steps, often leading to inconsistencies in scope and challenges in data transfer and rescaling.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Bioscience, Research and Early Development, Oncology, AstraZeneca, Cambridge, Cambridgeshire, UK.
A protocol for the preparation of tissue extracts for the targeted analysis ca. 150 polar metabolites, including those involved in central carbon metabolism, is described, using a reversed phase ion pair U(H)PLC-MS method. Data collection enabled in high-resolution mass spectrometry detection provides highly specific and sensitive acquisition of metabolic intermediates with wide range physicochemical properties and pathway coverage.
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