The fluoride (F), calcium (Ca) and phosphorous (P) content in two parts of the bone mandible, lumbar spine and femur of rats fed a low mineral diet and corresponding controls were determined by X-ray fluorescence. The anterior part of the mandible, including teeth was labeled as part I while the posterior part, including the mandibular head but excluding the teeth was designed as part II. The bone Ca, P and F content of part I was the same for the experimental and control groups. The Ca, P and F content of part II was decreased in the low-mineral diet group (p < 0.01). The values for F were considerably lower than in the controls. The lumbar spine and femur were used as whole bones and showed a significantly lower values for the three elements measured in the experimental group (p < 0.01). Stress or other stimuli from mandibular movement and occlusal force may be involved in preventing bone density decline, while density loss could be a result of lower physical stimulation, which may exert a greater effect than the level of fluoride in bones. It is possible that the absence of significant difference in fluoride levels in part I of the mandible may have been caused by its accumulation in teeth.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A self-assembled fluorescent nanoprobe recognized by FA1 site for specifically selecting HSA: Its applications in hemin detection, cell imaging and fluorescent tracing drug delivery.
Bioorg Chem
December 2024
Pancreas Center, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, State Key Laboratory of Druggability Evaluation and Systematic Translational Medicine, Tianjin Key Laboratory of Digestive Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China. Electronic address:
As naturally essential biomacromolecule, HSA has become diagnostic indicators for various diseases and universal carriers for anticancer drug delivery, therefore, fluorescence detection and labeling for HSA possess significant application value in the biomedical field. In this paper, hydrazide Schiff base fluorescent probe NDQC was designed and synthesized, which self-assembled into nanoparticles in aqueous solution system and demonstrated excellent selectivity and sensitivity towards HSA. Through displacement assay and molecular docking simulation, the binding of NDQC with HSA in FA1 site was demonstrated, thereby no obvious fluorescence signal presented for homologous protein BSA due to their structural differences in binding site.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Stanford University, Stanford, CA, USA.
Background: Recent studies suggest that iron and neuroinflammation are key components of Alzheimer's Disease (AD) pathology. Ferrous Fe can cause oxidative stress and cellular toxicity, but it is unknown to what extent Fe is elevated in AD, in particular with the hippocampus. To answer this question, we quantified iron oxidation state in frozen human brain hippocampi.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
USC Keck School of Medicine, Los Angeles, CA, USA.
Background: Human Apolipoprotein (APOE) has three isoforms, ε2, ε3, and ε4 among which ε4 (APOE4) confers the highest risk for late-onset Alzheimer's disease (AD). APOE4 is also the most prone to aggregate among APOE isoforms. Current evidence strongly suggests that APOE aggregation leads to neuronal dysfunction and eventually to AD.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
The UC Irvine Institute for Memory Impairments and Neurological Disorders (UCI MIND), Irvine, CA, USA.
Background: Brain deposits of amyloid-β (Aβ), one of the hallmark pathologies of Alzheimer disease (AD), are consistently present in people with Down syndrome (DS) after the age of 30 years. Positron emission tomography (PET) radioligands like [3H]Pittsburgh Compound-B (PiB) allow for visualizing Aβ accumulation in living people. In DS, the earliest and strongest PiB-PET retention is in the striatum, differing from late-onset AD.
View Article and Find Full Text PDFEnabling simultaneous photoluminescence spectroscopy and X-ray footprinting mass spectrometry to study protein conformation and interactions.
Anal Methods
January 2025
Molecular Foundry Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
X-ray footprinting mass spectrometry (XFMS) is a structural biology method that uses broadband X-rays for hydroxyl radical labeling to map protein interactions and conformation in solution. However, while XFMS alone provides important structural information on biomolecules, as we move into the era of the interactome, hybrid methods are becoming increasingly necessary to gain a comprehensive understanding of protein complexes and interactions. Toward this end, we report the development of the first synergetic application of inline and real-time fluorescent spectroscopy at the Advanced Light Source's XFMS facility to study local protein interactions and global conformational changes simultaneously.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!