A series of Tn5 transposases (Tnp's) with mutations at the conserved amino acid position W450, which was structurally predicted to be important for synapsis, have been generated and characterized. This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within the Tnp monomer that negatively regulate synaptic complex formation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2238198 | PMC |
| http://dx.doi.org/10.1128/JB.01488-07 | DOI Listing |
Publication Analysis
Top Keywords
synaptic complex
8
complex formation
8
tn5 synaptic
4
formation role
4
role transposase
4
transposase residue
4
residue w450
4
w450 series
4
series tn5
4
tn5 transposases
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!