Activation of natural killer-like T (NKT) cells with the CD1d ligand alpha-galactosylceramide enhances T-dependent humoral immune responses against coadministered T-dependent Ag. At present, there is little information on the mechanisms involved other than a dependence on CD1d expression by antigen-presenting cells and/or development of the NKT subset. We therefore tested the hypothesis that direct presentation of alpha-GC by B cells was required for NKT-enhanced Ab responses against T-dependent Ag. We reconstituted B cell-deficient microMT mice with B cells from C57Bl/6 donors or CD1d(-/-) donors before immunization with NP-KLH alone or NP-KLH mixed with alpha-GC. We made the surprising observation that B-cell expression of CD1d is absolutely required for the NKT-enhanced Ab response. Our data show that the mechanism by which NKT cells enhance humoral immune responses involves interaction with CD1d-expressing B cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234052 | PMC |
| http://dx.doi.org/10.1182/blood-2007-10-117309 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bioinformatics-based analysis of the role of immune-related genes in acute rejection after kidney transplantation and renal cancer development.
Medicine (Baltimore)
November 2024
Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Acute rejection (AR) is a common complication in the early stage after kidney transplantation. Some studies have shown that the occurrence of AR after kidney transplantation may further affect the development of tumors, and both AR and tumor development are related to immune cells and immune genes, so it is particularly important to diagnose the occurrence of AR at an early stage and to analyze the correlation between AR and tumors. In this study, we applied bioinformatics techniques for differential expression analysis and weighted gene co-expression network analysis analysis of AR patients to obtain differentially expressed genes and modular genes significantly associated with AR, respectively, so as to obtain their intersecting genes with immune-related genes; 21 intersecting genes were screened by lasso regression and Boruta algorithm to obtain the genes, and finally, the feature genes that were significantly associated with the dependent variable were further obtained by single-factor and multi-factor logistic regression.
View Article and Find Full Text PDFThe α glycolipid rules the NKT cell TCR.
J Exp Med
February 2025
La Jolla Institute for Immunology, La Jolla, CA, USA.
In this issue of JEM, Hosono et al. (https://doi.org/10.
View Article and Find Full Text PDFIdentification of α-galactosylceramide as an endogenous mammalian antigen for iNKT cells.
J Exp Med
February 2025
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Article Synopsis
- Invariant natural killer T (iNKT) cells are unique T cells that recognize lipid antigens through a molecule called CD1d, with α-galactosylceramide (α-GalCer) being the strongest known antigen.
- Researchers created a specialized system using supercritical fluid chromatography tandem mass spectrometry (SFC/MS/MS) to separate and identify different forms of hexosylceramide.
- Their findings revealed that α-GalCer, previously not found in mammals, is present in various biological fluids, representing the first identification of this potent antigen in mammalian systems.
CAR-iNKT cell therapy: mechanisms, advantages, and challenges.
Curr Res Transl Med
December 2024
Beijing Rongai Biotechnology Co., Ltd, 1st Floor, Building 29, No. 5 Kechuang East 2nd Street, Tongzhou District, Beijing 101100, China. Electronic address:
Article Synopsis
- CAR T-cell therapy has shown significant promise in treating blood cancers like B-ALL and multiple myeloma, leading to new approvals of CAR-T products that benefit many patients.
- However, its use in solid tumors is limited due to challenges like tumor microenvironments, variable antigen expression, and side effects.
- Invariant natural killer T (iNKT) cells, with their unique properties, are being investigated as a complement to CAR-T therapy, and researchers are exploring CAR technology to enhance iNKT cells’ effectiveness in targeting solid tumors.
Diverse NKT cells regulate early inflammation and neurological outcomes after cardiac arrest and resuscitation.
Sci Transl Med
December 2024
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
Neurological injury drives most deaths and morbidity among patients hospitalized for out-of-hospital cardiac arrest (OHCA). Despite its clinical importance, there are no effective pharmacological therapies targeting post-cardiac arrest (CA) neurological injury. Here, we analyzed circulating immune cells from a large cohort of patients with OHCA, finding that lymphopenia independently associated with poor neurological outcomes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!