Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2238116 | PMC |
http://dx.doi.org/10.1128/JCM.02009-07 | DOI Listing |
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