AI Article Synopsis
- The joggle mouse is a mutant strain of mice with ataxia, linked to an unknown genetic mutation derived from a specific chromosomal segment of the C3H/He strain.
- Researchers used the mouse genome database to analyze 127 DNA microsatellite markers that show differences between the C3H and C57BL/6J mouse strains and initially screened these markers in partial congenic mice.
- The study successfully mapped the joggle mutation to a specific interval on chromosome 6, between markers D6Mit104 and D6Mit336, using a more efficient method than traditional genetic linkage analysis.
Article Abstract
The joggle mouse is a recessive ataxic mutant carrying an unknown mutation in a C3H/He (C3H)-derived chromosomal segment. Taking advantage of the mouse genome database, we selected 127 DNA microsatellite markers showing heterozygosity between C3H and C57BL/6J (B6) and a first round of screening for the joggle mutation was performed on B6-jog/+ partial congenic mice (N4). We identified 4 chromosomal regions in which 13 microsatellite markers show heterozygosity between C3H and B6. Then, we analyzed the genotype of these 4 chromosomal regions in mice that showed the joggle phenotype and mapped the jog locus between markers D6Mit104 (111.4 Mb) and D6Mit336 (125.1 Mb) (an interval of 13.7 Mb) on chromosome 6. By using a partial congenic strain together with the mouse genome database, we successfully mapped the chromosomal localization of the jog locus much more efficiently than by conventional linkage analysis.
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| http://dx.doi.org/10.1538/expanim.56.389 | DOI Listing |
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