AI Article Synopsis
- A study on rhesus cytomegalovirus (RhCMV) genomes showed that a specific region (U(L)b') undergoes genetic changes similar to those in human cytomegalovirus (HCMV) after being repeatedly passed through cells.
- Researchers amplified the U(L)b' sequence from naturally infected rhesus macaques to determine its coding content, identifying 24 open reading frames (ORFs) that possibly encode proteins, with 10 related to HCMV and 15 to known RhCMV ORFs.
- The study also discovered three new alpha chemokine-like ORFs in this region, increasing the total alpha chemokine genes to six, with three showing significant sequence variation,
Article Abstract
A recent comparison of two rhesus cytomegalovirus (RhCMV) genomes revealed that the region at the right end of the U(L) genome component (U(L)b') undergoes genetic alterations similar to those observed in serially passaged human cytomegalovirus (HCMV). To determine the coding content of authentic wild-type RhCMV in this region, the U(L)b' sequence was amplified from virus obtained from naturally infected rhesus macaques without passage in vitro. A total of 24 open reading frames (ORFs) potentially encoding >99 amino acid residues were identified, 10 of which are related to HCMV ORFs and 15 to previously listed RhCMV ORFs. In addition, the analysis revealed a cluster of three novel alpha chemokine-like ORFs, bringing the number of predicted alpha chemokine genes in this region to six. Three of these six genes exhibit a high level of sequence diversity, as has been observed for the HCMV alpha chemokine gene UL146.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766279 | PMC |
| http://dx.doi.org/10.1016/j.virol.2007.10.040 | DOI Listing |
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