Objective: To assemble the full-length of human resistin gene in vitro by using oligonucleotides and to construct its eukaryotic expression vector.
Methods: According to the gene sequence of resistin (GenBank: AF323081), 10 oligonucleotides were designed and synthesized, followed by a touch down PCR to assemble the full-length gene. The PCR products were cloned into pSecTag2B vector and confirmed by sequencing.
Results: The band of PCR products and gene sequencing showed the insert fragment in pSecTag2B vector was identical to that as designed.
Conclusion: The full-length of human resistin coding sequence was successfully assembled and amplified by touch down PCR, and a resistin-expressing eukaryotic vector was constructed.
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| http://dx.doi.org/10.3785/j.issn.1008-9292.2007.06.012 | DOI Listing |
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