AI Article Synopsis

  • Genetic diversity among 9 native Chinese cattle breeds and 3 introduced ones was analyzed using 12 microsatellite DNA markers through a specific PCR method.
  • Various genetic metrics, like mean heterozygosity and genetic distances, were calculated, leading to a clear clustering of the breeds into four distinct groups based on their geographical origins.
  • The findings highlight the importance of preserving and utilizing Chinese native cattle breeds, contributing to their genetic conservation efforts.

Article Abstract

The genetic diversity of 9 Chinese native cattle breeds and 3 introduced breeds were analyzed using 12 microsatellite DNA markers recommended by the Food and Agriculture Organization of the United Nations (FAO) and the International Society of Animal Genetics (ISAG) through fluorescence-multiple PCR. According to the allele frequencies of 12 microsatellite locus, mean heterozygosity (H), polymorphism information content (PIC) , DA and DS genetic distances were calculated for each breed. Cluster analysis based on UPGMA method indicated that 12 breeds were clustered into four groups. Group I belonged to the southern-China cattle including Enshi, Liping, Zhaotong and Chuannan Mountainous; Group II belonged to the central-China cattle including Jiaxian Red and Zaosheng, Pinglu Mountainous; Group III belonged to the northern-China cattle including Yanbian and Changbai; Group IV consisted of foreign breeds including Germany Yellow, Simmental and Charolais. The results may provide an academic basis for preservation and utilization of Chinese native cattle breeds.

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