AI Article Synopsis
- Three hybridoma cell lines (2C6, 5B7, and 12G9) were created by fusing mouse myeloma cells with spleen cells from BALB/C mice immunized against Cymbidium mosaic virus (CymMV).
- The monoclonal antibodies produced exhibit strong specificity for CymMV, with ascitic fluid titres reaching up to 10^(-6) in I-ELISA tests.
- The antibodies were effective in an ACP-ELISA technique, successfully detecting as little as 0.487 ng of purified CymMV and confirming its presence in field orchid tissues.
Article Abstract
Three hybridoma cell lines, 2C6, 5B7 and 12G9, secreting monoclonal antibodies (McAbs) against Cymbidium mosaic virus (CymMV) were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/ C immunized by the CymMV particles. The three McAbs could specifically react with CymMV. The titres of ascitic fluids of two McAbs are up to 10(-6) in I-ELISA. Isotypes and subclasses of the the three McAbs belong to IgG1. Isotypes of light strains of the three McAbs all belong to kappa. They were used in antigen-coated plate (ACP)-ELISA for CymMV detection, and ACP-ELISA could successfully detect 0.487 ng of purified CymMV or virus in plant sap diluted 1:10240. The presence of CymMV in field Orchids tissues was investigated with ACP-ELISA.
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