AI Article Synopsis
- Human seminal plasma (HSP) allergy is rare but can lead to severe reactions, often appearing during or after the first sexual encounter, with prostate-specific antigen (PSA) being identified as a key allergen.
- Researchers investigated potential IgE cross-reactivity between allergens in dog epithelium and HSP in patients with dog allergies, using various testing methods.
- The study found that 24% of patients with dog allergies had IgE that recognized PSA, and preincubation with dog dander completely inhibited IgE-binding, confirming a cross-reactivity between dog allergens and PSA.
Article Abstract
Background: Human seminal plasma (HSP) allergy is uncommon, with symptoms ranging from vulvovaginal pruritus to life-threatening anaphylaxis. Although several seminal plasma allergens have been reported and their molecular masses have been estimated to range between 12 and 75 kd, the prostate-specific antigen (PSA) has recently been identified as a causative allergen. Given that in a large number of cases symptoms appeared during or after the first intercourse, a cross-reactivity phenomenon might be implicated.
Objective: We sought to assess the presence of IgE cross-reactivity among proteins from dog epithelium and HSP and to attempt to identify the allergens involved.
Methods: Forty-one patients with dog epithelium allergy were selected. One of them experienced anaphylaxis in contact with her husband's seminal plasma. Skin prick tests, serum specific IgE measurements, SDS-PAGE immunoblotting, and inhibition tests were performed to study the pattern of IgE-binding proteins and the potential cross-reactivity between HSP and dog epithelium. Mass spectrometry was carried out to identify the protein involved in allergy reactions.
Results: Twenty-four percent of the sera from patients with dog epithelium allergy recognized an IgE-binding band of 28 kd in HSP immunoblotting. Mass spectrometry identified this band as the PSA. SDS-PAGE immunoblotting-inhibition showed a complete IgE-binding inhibition when sera from these patients were preincubated with dog dander extract.
Conclusions: IgE cross-reactivity among proteins from dog dander and human PSA is demonstrated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jaci.2007.10.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Interplay of geometry and mechanics in epithelial wound healing.
Phys Rev E
November 2024
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India.
Wound healing is a complex biological process critical for maintaining an organism's structural integrity and tissue repair following an infection or injury. Recent studies have unveiled the mechanisms involving the coordination of biochemical and mechanical responses in the tissue in wound healing. In this article, we focus on the healing property of an epithelial tissue as a material while the effects of biological mechanisms such as cell proliferation, tissue intercalation, cellular migration, cell crawling, and filopodia protrusion is minimal.
View Article and Find Full Text PDFAn actin bracket-induced elastoplastic transition determines epithelial folding irreversibility.
Nat Commun
December 2024
Nano Life Science Institute, Kanazawa University, Kanazawa, Japan.
During morphogenesis, epithelial sheets undergo sequential folding to form three-dimensional organ structures. The resulting folds are often irreversible, ensuring that morphogenesis progresses in one direction. However, the mechanism establishing folding irreversibility remains unclear.
View Article and Find Full Text PDFMass Spectrometry Imaging Distinguishes Biliary Toxicants on the Basis of Cellular Distribution.
Toxicol Pathol
December 2024
Abbvie Inc., North Chicago, Illinois, USA.
Mass spectrometry imaging (MSI) was used to investigate and provide insights into observed biliary pathology found in dogs and rats after administration of two different compounds. Both compounds were associated with peribiliary inflammatory infiltrates and proliferation of the bile duct epithelium. However, MSI revealed very different spatial distribution profiles for the two compounds: Compound A showed significant accumulation within the bile duct epithelium with a much higher concentration than in the parenchymal hepatocytes, while Compound T exhibited only a slight increase in the bile duct epithelium compared to parenchymal hepatocytes.
View Article and Find Full Text PDFImmunohistochemical assessment of ERM proteins (ezrin, radixin, moesin) in the ovaries of different species.
Tissue Cell
November 2024
Department of Biomedical Sciences and Pathobiology, Center of Pathobiology, University of Veterinary Medicine, Vienna, Austria; VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria. Electronic address:
Article Synopsis
- Ezrin, radixin, and moesin (ERM) proteins connect membrane proteins to the actin cytoskeleton, but their role in healthy reproductive tissues is under-researched.
- A study used immunohistochemistry to analyze the distribution of these proteins in ovaries from various animal species, including mice, dogs, and pigs.
- Findings revealed differences in localization and expression of ERM proteins across species and ovarian structures, which could enhance our understanding of their physiological roles in female reproduction.
Effect of Subconjunctival Injection of Canine Adipose-Derived Mesenchymal Stem Cells on Canine Spontaneous Corneal Epithelial Defects.
Animals (Basel)
November 2024
Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Spontaneous chronic corneal epithelial defects (SCCEDs) are characterized by nonadherent corneal epithelium leading to poor attachment to the corneal stroma. The objective of this study was to characterize corneal outcomes concurrently with the quantification of tumor necrosis factor-alpha (TNF-α) and vascular endothelial growth factor-A (VEGF-A) in tear fluid after the subconjunctival injection of canine adipose-derived mesenchymal stem cells (cAD-MSCs) in canine SCCEDs. Ten eyes with SCCEDs, which were nonresponsive to two rounds of diamond burr debridement, were included in this study.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!