A performant reagentless electrochemiluminescent (ECL) detection system for H(2)O(2) is presented, based on an electropolymerized polyluminol film prepared under near-neutral conditions. Such an original polyluminol electrodeposition is reported for the first time and on a screen-printed electrode (SPE) surface. Electropolymerized luminol acts as an active luminophore of the electrochemiluminescent reaction, as the monomer does. Polymerization conditions have been optimized in order to obtain the best ECL responses to H(2)O(2). By performing electrodeposition in a potentiostatic mode, at 425 mV vs. Ag|AgCl, in 0.1 mol L(-1) phosphate/0.1 mol L(-1) KCl pH 6 and 1 mmol L(-1) luminol, with a total charge of 0.5 mC, the linear range for H(2)O(2) detection extends from 7.9 x 10(-8) mol L(-1) to 1.3 x 10(-3) mol L(-1). Such performant disposable reagentless easy-to-use miniaturized systems based on SPEs should be applicable to the electrochemiluminescent detection of many oxidase-substrate compounds.
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