AI Article Synopsis
- RNA interference (RNAi) has potential as a therapeutic method to silence disease-related genes and has been effectively used in genomic research.
- Attenuated S. typhimurium can deliver short hairpin RNA (shRNA) vectors into mammalian cells, successfully inducing RNAi in lab settings and live organisms.
- When administered orally, S. typhimurium carrying shRNA targeting the bcl2 gene led to significant gene silencing in melanoma cells, resulting in delayed tumor growth and increased survival in mouse models, indicating a promising cancer treatment method.
Article Abstract
RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi in vitro and in vivo. Upon oral administration, S. typhimurium carrying shRNA-expressing vectors targeting bcl2 induced significant gene silencing in murine melanoma cells that led to a remarkably delayed tumor growth and prolonged survival in the mouse model. These results suggest that bacteria mediated RNAi may be a new potent approach to the treatment of cancers.
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| http://dx.doi.org/10.4161/cbt.7.1.5195 | DOI Listing |
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