AI Article Synopsis
- Tritrichomonas foetus causes trichomoniasis in cattle, leading to significant economic losses in the beef and dairy industries due to issues like abortion and infertility.
- Soluble DNases extracted from T. foetus were found to be glycoproteins, indicating their potential as therapeutic targets, and their activity was influenced by metal ions such as manganese and zinc.
- The DNases function optimally at a pH of 4, have a molecular weight of 160 kDa, and can degrade DNA from various biological sources, but show little to no activity on RNA.
Article Abstract
Tritrichomonas foetus is the causative agent of trichomoniasis. In cattle, infection results in economic losses to the beef and dairy industries due to abortion and infertility. Soluble DNases of T. foetus that play a role in pathogenesis and are potential therapeutic targets, were extracted and purified utilising lectin affinity chromatography. The DNases were bound to and eluted from Concanavalin A (Con A)-sepharose indicating that they are glycoproteins with alpha-linked mannose or glucose residues. The nature of the glycans carried on the eluted proteins in the fraction containing DNase activity was assessed using an enzyme-linked lectin assay. The lectin binding studies predict the presence of both N- and O-type glycans. Manganese was a potent (33%) activator of the DNase(s) whereas zinc inhibited enzyme activity by approximately 66%. The DNase(s) had a pH optimum of 4 and a molecular weight of 160 kDa. The DNase(s) were able to completely degrade DNA from animal, plant, fungal, yeast and bacterial sources, but did not significantly degrade RNA.
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| http://dx.doi.org/10.1016/j.ijpara.2007.10.012 | DOI Listing |
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