AI Article Synopsis
- A partial pseudo-ternary phase diagram was developed for a system involving cethyltrimethylammonium bromide, isooctane, hexanol, butanol, and potassium phosphate buffer, showing the equilibrium of reverse micelles with solvent.
- *Different two-phase systems of reverse micelles were created for enzyme extraction, focusing on glucose-6-phosphate dehydrogenase and xylose reductase derived from Candida guilliermondii yeast.
- *The findings suggest that varying the composition of the micelles allows for selective extraction of the enzymes, which can help in optimizing enzyme extraction processes.*
Article Abstract
A partial pseudo-ternary phase diagram has been studied for the cethyltrimethylammonium bromide/isooctane:hexanol:butanol/potassium phosphate buffer system, where the two-phase diagram consisting of the reverse micelle phase (L2) in equilibrium with the solvent is indicated. Based on these diagrams two-phase systems of reverse micelles were prepared with different compositions of the compounds and used for extraction and recovery of two enzymes, and the percentage of enzyme recovery yield monitored. The enzymes glucose-6-phosphate dehydrogenase (G6PD) and xylose redutase (XR) obtained from Candida guilliermondii yeast were used in the extraction procedures. The recovery yield data indicate that micelles having different composition give selective extraction of enzymes. The method can thus be used to optimize enzyme extraction processes.
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| http://dx.doi.org/10.1016/j.jchromb.2007.10.046 | DOI Listing |
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