AI Article Synopsis
- A new two-step triplex PCR test was created to find specific methicillin resistance genes in Staphylococcus aureus bacteria and to identify the species at the same time.
- The test showed that all 105 S. aureus isolates had femA and nuc genes, but only 44 of the clinical isolates were positive for the mecA gene, with no foodborne isolates testing positive.
- The PCR results matched well with PBP2a latex agglutination tests and oxacillin susceptibility tests, showing a correlation of 98-99%.*
Article Abstract
A two-step triplex PCR assay targeting the mecA, femA, and nuc genes was developed for the detection of methicillin resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. The triplex PCR revealed the presence of the femA and nuc genes in all the S. aureus isolates examined (n=105). Forty-four clinical isolates were mecA positive and no foodborne isolates were mecA positive. The PCR results had a 98 or 99% correlation with the results of PBP2a latex agglutination tests or oxacillin susceptibility tests, respectively.
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