A physical map of the Casphalia extranea densovirus genome (CeDNV) was constructed. The size of the intact viral genome was estimated to be 4.9 kilobases or 1.6 MDa (single strand). The double-stranded CeDNV genomic DNA was cleaved with 26 restriction endonucleases and 20 restriction sites were mapped on the genome. The CeDNV DNA restriction map was compared to those of other densoviruses. Southern blotting hybridization experiments failed to reveal any homology between the genomes of CeDNV and Junoniacoenia densovirus (JcDNV).
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http://dx.doi.org/10.1016/0923-2516(91)90072-b | DOI Listing |
Curr Res Parasitol Vector Borne Dis
January 2025
Western University of Health Sciences, 200 Mullins Dr, Lebanon, OR, 97355, USA.
snails are intermediate hosts for schistosome parasites, which cause morbidity and mortality in humans worldwide. We aimed to determine the mating system of , a hermaphroditic vector of schistosomiasis in the African Great Lakes, with the goal of informing the design of genetic studies such as linkage mapping to improve genome assembly and genetic association studies to identify snail resistance genes. To determine the relative rates of outcrossing selfing, we assayed the progeny of experimental crosses of snails in the laboratory using a PCR and restriction enzyme digest to determine snail genotype and parentage.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA.
While all native tRNAs undergo extensive post-transcriptional modifications as a mechanism to regulate gene expression, mapping these modifications remains challenging. The critical barrier is the difficulty of readthrough of modifications by reverse transcriptases (RTs). Here we use Induro-a new group-II intron-encoded RT-to map and quantify genome-wide tRNA modifications in Induro-tRNAseq.
View Article and Find Full Text PDFClin Genet
January 2025
Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Brazil.
Silver-Russell Syndrome (SRS) is a genetic disorder characterized by intrauterine and postnatal growth restriction. Most cases are caused by an imprinting error either with hypomethylation of the Imprinted Control Region 1 at 11p15.5, or maternal uniparental disomy of chromosome 7.
View Article and Find Full Text PDFFront Plant Sci
January 2025
Institute of Biodiversity, Hunan Academy of Forestry, Changsha, China.
(L.) Sw. is a valuable ornamental plant in the genus , family Orchidaceae, with high economic and ecological significance.
View Article and Find Full Text PDFFront Microbiol
January 2025
Department of Intensive Care Unit, The Peoples Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.
Background: Leptospirosis is an acute zoonotic disease caused by pathogenic , primarily transmitted to humans through contact with water or soil contaminated by the bacteria. It is globally distributed, with heightened prevalence in tropical regions. While prior studies have examined the pathophysiology, epidemiology, and risk factors of leptospirosis, few have explored trends and emerging topics in the field.
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