Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based detection technology or in cell.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/nass/nrm012 | DOI Listing |
Publication Analysis
Top Keywords
dual-labeled oligonucleotide
8
design dual-labeled
4
oligonucleotide probes
4
probes snps
4
snps genotyping
4
genotyping effort
4
effort designing
4
designing base-discriminating
4
base-discriminating fluorescence
4
fluorescence nucleosides
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!