AI Article Synopsis
- Glycosylphosphatidylinositols (GPIs) are glycolipids that attach proteins to eukaryotic cells and are important for expressing surface antigens from parasitic protozoa in different systems.
- The prior method of baculovirus infection significantly reduced GPI-anchor synthesis, affecting protein expression levels.
- This study introduces a new technique to improve GPI-anchor protein expression in insect cells by using a baculovirus that enhances the activity of a key enzyme in the GPI biosynthetic process.
Article Abstract
Glycosylphosphatidylinositols (GPIs) constitute a class of glycolipids that have various functions, the most basic being to attach proteins to the surface of eukaryotic cells. GPIs have to be taken into account, when expressing surface antigens from parasitic protozoa in heterologous systems. The synthesis of the GPI-anchors was previously reported to be drastically decreased to almost background level following baculovirus infection. Here we describe a new method to express GPI-anchor proteins in insect cells relying on using of a supplementary baculovirus construct that overexpresses the N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the enzyme catalyzing the second step in the GPI biosynthetic pathway.
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| http://dx.doi.org/10.1016/j.bbrc.2007.11.026 | DOI Listing |
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