AI Article Synopsis

Article Abstract

Identification and characterization of nucleotide substitutions in DNA sequences for single nucleotide polymorphism or point mutation detection can be a time consuming and sometimes inaccurate process, particularly in relatively low-throughput situations where fully automated solutions may not be appropriate. SeqDoC provides a simple web-based application to simplify this identification process, by using direct subtractive comparison of the raw sequence traces to highlight differences characteristic of nucleotide substitutions. Sequencing artefacts, such as variable peak separation and signal strength, are compensated for with moving window normalisation functions, whereas the signal to noise ratio of the comparison trace is greatly enhanced by applying an algorithm to emphasise features associated with nucleotide substitutions. Analysis of the output is simple and intuitive, permitting rapid identification of points of difference between the reference and test sequence traces.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-59745-515-2_22DOI Listing

Publication Analysis

Top Keywords

nucleotide substitutions
16
rapid identification
8
single nucleotide
8
sequence traces
8
nucleotide
5
identification single
4
substitutions
4
substitutions seqdoc
4
seqdoc identification
4
identification characterization
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!