[Construction of tissue engineered gingiva with gingival epithelia and acellular dermal matrix].

Objective: To culture human gingival epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).

Methods: Human gingival epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.

Results: Human gingival epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.

Conclusion: Tissue engineered gingiva may be constructed with human gingival epithelia and ADM in vitro.