Purpose: To investigate the diffusion effect of calcium hydroxide in periapical simulation of the diffusion model.
Methods: Pipetman pipette tips, plastic syringes and BHI agar were prepared to be periapical simulation of the diffusion model. 120 simulation models were divided into three groups. Group 1: 100 microl 20% calcium hydroxide suspension were sealed in pipette tips; group 2: 100 microl 90% calcium hydroxide cataplasm were sealed in; group 3: 100 microl calcium hydroxide points were sealed in. By different apical aperture (15#,25#,40#,80#), each group was divided into four subgroups (each subgroup contained 10). Preparation of 12 other simulation models sealed with distilled water were as a control. pH values and Ca(2+) concentration were measured by PH analyzer and calcium analyzer respectively. The data was collected to establish a database, using SPSS13.0 software package, randomized block design ANOVA was performed.
Results: The average pH value (8.26, S=0.86) of group 1 was significantly higher than that in group 2 (7.96, S=0.702) and Group 3 (7.83, S=0.59) (P<0.05), but no significant difference was found between group 2 and group 3. The average calcium concentration of group 1 (29.87 ppm. S=10.76) was significantly higher than that in group 2 (24.62 ppm. S=10.40) and group 3(16.42 ppm, S=5.70). There were significant differences among the three groups (P<0.05). The results also showed that the average pH value and the average calcium concentration were increased with apical aperture. There were significant differences between each group (P<0.05).
Conclusion: The diffusion effect of calcium hydroxide suspension in periapical simulation of the diffusion model is better than calcium hydroxide cataplasm and calcium hydroxide points; the diffusion effect is also proportional to the apical aperture. Supported by Key Research Project of Science and Technology Bureau of Sichuan Province (Grant No.05SG022-007).
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