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Microbial single-cell applications under anoxic conditions.
Appl Environ Microbiol
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Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research (NIOZ), Den Burg, the Netherlands.
The field of microbiology traditionally focuses on studying microorganisms at the population level. Nevertheless, the application of single-cell level methods, including microfluidics and imaging techniques, has revealed heterogeneity within populations, making these methods essential to understand cellular activities and interactions at a higher resolution. Moreover, single-cell sorting has opened new avenues for isolating cells of interest from microbial populations or complex microbial communities.
View Article and Find Full Text PDFOligo cyc-DEP: On-chip cyclic immunofluorescence profiling of cell-derived nanoparticles.
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Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, Portland, Oregon, USA.
We present a follow-on technique for the cyclic-immunofluorescence profiling of suspension particles isolated using dielectrophoresis. The original lab-on-chip technique ("cyc-DEP" [cyclic immunofluorescent imaging on dielectrophoretic chip]) was designed for the multiplex surveillance of circulating biomarkers. Nanoparticles were collected from low-volume liquid biopsies using microfluidic dielectrophoretic chip technology.
View Article and Find Full Text PDFSchistosomiasis diagnosis: Challenges and opportunities for elimination.
PLoS Negl Trop Dis
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Centre for Research in Infectious Diseases, College of Graduate Studies and Research, Mount Kenya University, General Kago Rd, Thika, Kenya.
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- * Effective surveillance using accurate diagnostics is crucial for monitoring schistosomiasis control efforts, with microscopy still being the gold standard despite its limitations.
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Q3 lab-on-chip real-time PCR for the diagnosis of infection in dogs.
J Clin Microbiol
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Department of Veterinary Medicine, University of Bari, Bari, Italy.
Leishmaniasis is a vector-borne disease caused by many spp. which infect humans and other mammalian hosts. is the main agent of canine leishmaniasis (CanL) whose diagnosis is usually confirmed by serological and molecular tests.
View Article and Find Full Text PDFAlternative nano-lithographic tools for shell-isolated nanoparticle enhanced Raman spectroscopy substrates.
Nanoscale
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BIOS Lab on Chip Group, Mesa+ Institute of Nanotechnology, University of Twente, The Netherlands.
Chemically synthesized metal nanoparticles (MNPs) have been widely used as surface-enhanced Raman spectroscopy (SERS) substrates for monitoring catalytic reactions. In some applications, however, the SERS MNPs, besides being plasmonically active, can also be catalytically active and result in Raman signals from undesired side products. The MNPs are typically insulated with a thin (∼3 nm), in principle pin-hole-free shell to prevent this.
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